When should I use an Objective Heater

You should use an objective heater, if you:

• Perform Oil Immersion Imaging with a Top Stage Incubator
• Use dipping lens with an upright Top Stage Incubator

Why should I use an Objective Heater?

Because, if you don't, sample temperature will drop due to oil contact with the cold objective, as shown in the following graph.

Step 1: Objective not in contact with Petri 2; Objective Heater Off.

Step 2: Objective in oil contact with Petri 2; Objective Heater Off.

Step 3: Objective in oil contact with Petri 2; Objective Heater on, Offset value 5.3°C.

During the self calibration routine, the offset value of the objective heater is progressively increased, until T2 reaches the desired set point temperature of 37.0°C.

Figure 3 reports the profile of T1, T2, and of Objective Heater Temperature during the experimental campaign described above.