Enclosure
The microscope enclosure creates a large volume around the microscope in which temperature is controlled by recirculating warm air. It is made of polycarbonate panels and encloses microscope stage, nosepiece and objectives. It doesn't affect focus stability. All electronics including EMCCDs, spinning disk confocal etc. are left outside of the enclosure avoiding exposure to 37°C.
The enclosure closely follows the microscope profile and is supported by posts resting on the vibration isolation table.
Features
- Temperature range: from 3°C above ambient to 45°C
- Temperature accuracy on sample: 0.1°C
- Models for any inverted / upright microscope
- Sliding doors on the front and side panels allow full and easy access to the microscope
- Removable front panel with turn-to-open hinges
- Optional black panels for imaging in dark environment- can be added at any time after purchase
- An LED light with foot pedal and dimmer allows illumination inside the enclosure
- Add a Micro - Environmental Chamber for Humidity and CO2 (and / or O2) control: models available for any XY stage and Piezo insert
- CO2 (and / or O2) levels set with Digital (Bold Line) or Manual Gas Controllers
- Humidity: Vibration Free Humidity Module or Active Humidity Controller
Need more space ?
Take the front panel off !
Fluorescence ?
Make the enclosure dark !
Family picture !
- Enclosure
- Temperature Controller
- Gas Chamber
- CO2 Digital Controller
- Vibration Free Humidity Module
H201-LG-DLS
The H201-LG-DLS Gas Chamber connects to Bold Line Temperature, Gas and Humidity Controllers and allows to perform time lapse imaging using the Leica Digital Light Sheet on living samples by controlling Temperature, Humidity and Gas concentration.
The Gas Chamber fits into the Super Z Galvo stage and features a lid to accommodate the TwinFlect mirror device. The warm air circulating in the enclosure warms up the detection objectives Leica HC APO L 10x/0.3 W and Leica HC FLUOTAR L 25x/0.95 W.
- 35/60 mm Petri dishes
- Compatible with Leica DLS module, it allows usage of illumination and detection objectives
- Includes an objective collar for detection objectives Leica HC APO L 10x/0.3 W and Leica HC FLUOTAR L 25x/0.95 W
- Uniformly heated by means of embedded electric resistances and covered with a temperature controlled lid
- Spring locks hold Petri dishes, in correct position inside the chamber
- Perfusion holes: 12 channels for 2.0 mm O.D. tubings are available
- The chamber screws onto Super Z galvo stage
- Chamber weight, 130 g.
- To control the humidity into the chamber humidity module is recommended: HM-ACTIVE
Available Inserts
| 1x35-DLS | #1 35mm Petri-dish holder |   |
| 1x60-DLS | #1 60mm Petri-dish holder |  |
Temperature Unit
Okolab Cage Incubator employs a Temperature Unit that generates warm air to raise the temperature inside the enclosure.
Superior accuracy is guaranteed by the interplay of four sensors: three for temperature and one for flow rate. The temperature sensors monitor temperature inside the enclosure, inside the Gas Micro-Environmental Chamber and inside the Air Heater. The flow rate sensor controls the rate at which warm air enters the enclosure and ensures smooth operation and fast heating. A proprietary smart algorithm determines the minimum flow rate necessary to maintain the controlled temperature at the set point value.
Okolab Cage Incubation System has both Enclosure (Chamber) Feedback mode as well as Sample Feedback mode as standard features. The end user can select the temperature sensor driving feedback to the controller.
Features
- Temperature range: from 3°C above ambient to 45°C
- Temperature accuracy: 0.1°C
- # 3 Temperature sensors: in the enclosure; in the gas chamber; inside the air heater
- # 1 Flow rate sensor inside the air heater
- Operated via touch screen interface OKO-TOUCH
- Double air input and output ensures best temperature uniformity inside the enclosure
- Low energy consumption thanks to warm air recirculation
- Filtering Unit - Optional
- Compatible with SMART BOX data logger - Optional
- Integrated in LAS X
Touch Pad Interface
Beautifully designed, simple to use and very compact.
OKO-TOUCH is all you need to operate the system.
CO2 - O2 CONTROLLERS
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and or O2 accuracy is ±0.1% with adjustable total output flow rate.
A vibration free humidity module is typically used with a Cage Incubation System. When Humidity control with feedback is required, the Active Humidity Controller can be connected to the Bold Line Gas Controller.
Features
- CO2 accuracy: 0.1% (see Controller page for more info)
- O2 accuracy: 0.1% (see Controller page for more info)
- Touch Screen Interface - OKO-TOUCH
- Output Flow Rate: adjustable from OKO-TOUCH
- Compatible with Active Humidity Controller
- Programmable CO2 (and /or) O2 cycles
- Compatible with SMART BOX for data logging and remote operation - Optional
- Data Logging via OKOLAB Data Log Software - Optional
- Compatible with LAS X
Preventing evporation?
Use the best Humidifier on the market: ACTIVE HUMIDITY CONTROLLER
It connects to the Gas Controller and shows up in OKO-TOUCH
Hypoxia and / or Hyperoxia ?
Oxygen control is our pride.
Check out the available solutions in the Controller List
Multiple units ? Be Smart - go central
One controller feeds up to 10 microscope incubators.
Microscopes can be in different rooms.
Available Bold Line Digital Controllers
| CO2 | O2 | Accuracy | Outlet P, barg (psi) | |
|---|---|---|---|---|
| CO2-Unit-BL | 0-18% | - | ±0.1% | 0 |
| CO2-Unit-BL-CP2 (multi-user centralized controller) | 0-10% | - | ±0.1% | 1.5 (22) |
| O2-Unit-BL [1-20] | - | 1-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10;1-18] | 0-10% | 1-18% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-20;1-95] | 0-20% | 1-95% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10; 0-1] | 0-10% | 0-1% | ±0.1% | 0 |
CO2 - O2 MANUAL GAS MIXERS
These gas mixers employ accurate floating ball flow meters to mix gas AIR and CO2 streams. The desired gas concentration is achieved by measuring the individual flow rated prior to mixing.
For Hypoxia or Hyperoxia applications, a third flow meter if added to measure N2 or O2 flow rate.
No feedback is present on gas concentration.
Features
- 2GF-MIXER mixes AIR with 100% CO2
- CO2 range: 0-15%; accuracy ±1%
- 3GF-MIXER-HYPOXIA mixes AIR, CO2 and N2
- CO2 range: 0-15%, accuracy ±1%
- O2 range: 2-20%, accuracy ±1%
- 3GF-MIXER-HYPEROXIA mixes AIR, CO2 and O2
- CO2 range: 0-15%, accuracy ±1%
- O2 range: 40-85%, accuracy ±1%
- Filtering device: PTFE membrane with 0.2 mm pores
- Compatible with OKO-AP, if compresses air is not available
- For Humidity Control, add Vibration Free Humidity Module
- Not compatible with Active Humidity Controller
Working with Premixed gas ?
All you need is a single flow meter to regulate the flux
Hypoxia and / or Hyperoxia ?
3GF-MIXER is a simple and cost effective solution also for these application.
No Compressed Air ?
Use AIR-PUMP to feed air into the Manual Gas Mixers
Available Manual Gas Mixers
| 2GF MIXER | 0-20% | - | 0 |
| 3GF MIXER-HYPOXIA | 0-15% | 2-20% | 0 |
| 3GF MIXER-HYPEROXIA | 0-15% | 40-85% | 0 |
VIBRATION FREE HUMIDITY MODULE
The Cage Incubator is equipped with a vibration-free gas humidifier which employes a semi-permeable membrane. Humidification occurs without bubbling to ensure vibration-free operation.
A water trap is located between the humidifier and the gas chamber preventing any water droplet from accidentally reaching the sample.
Features
- Gas is humidified through a semi-permeable membrane
- No bubbling, i.e. no vibrations
- Fits inside the enclosure
- Re-filler without opening the enclosure and without perturbing the sample
- If active control of humidity is needed, see HM-ACTIVE
OKO-TOUCH
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
It allows to:
- Turn all Bold Line Controllers on/off
- Define set point values
- Set flow rates of the Temperature Unit
- Set flow rates gas controller
- Define gas cycles of gas controller
- Select sample or enclosure feedback mode
- Display all set points and measured parameters
- Run self calibration procedures
SMART BOX
Smart Box connects to the Bold Line Controller and provides the following features:
Features
- Data Logging
- Remote Operation of the incubator via Internets, Tablet or SmartPhone
- Video Streaming server
- Remote assistant
