Enclosure
The microscope enclosure creates a large volume around the microscope in which temperature is controlled by recirculating warm air. It is made of polycarbonate panels and encloses microscope stage, nosepiece and objectives. It doesn't affect focus stability. All electronics including EMCCDs, spinning disk confocal etc. are left outside of the enclosure avoiding exposure to 37°C.
The enclosure closely follows the microscope profile and is supported by posts resting on the vibration isolation table.
Features
- Temperature range: from 3°C above ambient to 45°C
- Temperature accuracy on sample: 0.1°C
- Models for any inverted / upright microscope
- Sliding doors on the front and side panels allow full and easy access to the microscope
- Removable front panel with turn-to-open hinges
- Optional black panels for imaging in dark environment- can be added at any time after purchase
- An LED light with foot pedal and dimmer allows illumination inside the enclosure
- Add a Micro - Environmental Chamber for Humidity and CO2 (and / or O2) control: models available for any XY stage and Piezo insert
- CO2 (and / or O2) levels set with Digital (Bold Line) or Manual Gas Controllers
- Humidity: Vibration Free Humidity Module or Active Humidity Controller
Need more space ?
Take the front panel off !
Fluorescence ?
Make the enclosure dark !
Family picture !
- Enclosure
- Temperature Controller
- Gas Chamber
- CO2 Digital Controller
- Vibration Free Humidity Module
Temperature Unit
Okolab Cage Incubator employs a Temperature Unit that generates warm air to raise the temperature inside the enclosure.
Superior accuracy is guaranteed by the interplay of four sensors: three for temperature and one for flow rate. The temperature sensors monitor temperature inside the enclosure, inside the Gas Micro-Environmental Chamber and inside the Air Heater. The flow rate sensor controls the rate at which warm air enters the enclosure and ensures smooth operation and fast heating. A proprietary smart algorithm determines the minimum flow rate necessary to maintain the controlled temperature at the set point value.
Okolab Cage Incubation System has both Enclosure (Chamber) Feedback mode as well as Sample Feedback mode as standard features. The end user can select the temperature sensor driving feedback to the controller.
Features
- Temperature range: from 3°C above ambient to 45°C
- Temperature accuracy: 0.1°C
- # 3 Temperature sensors: in the enclosure; in the gas chamber; inside the air heater
- # 1 Flow rate sensor inside the air heater
- Operated via touch screen interface OKO-TOUCH
- Double air input and output ensures best temperature uniformity inside the enclosure
- Low energy consumption thanks to warm air recirculation
- Filtering Unit - Optional
- Compatible with SMART BOX data logger - Optional
- Integrated in third party software: Nikon NIS Elements; Metamorph; Micromanager
Touch Pad Interface
Beautifully designed, simple to use and very compact.
OKO-TOUCH is all you need to operate the system.
Enclosure Temperature Sensor
It measures air temperature inside the enclosure.
Sample Temperature Sensor?
It measures the temperature in a reference well near the sample.
It ensures Temperature stability even if the doors of the enclosure are temporarely opened.
GAS CHAMBER
The micro-environmental Gas Chamber fits on the microscope stage and hosts the sample. The Gas Chamber is located inside the enclosure and at the set point temperature - typically 37°C. The purpose for the Gas Chamber is to create a small volume surrounding the sample and containing humidified gas of the desired concentration.
When operating the Temperature Unit in Sample Feedback Mode, introduce the provided temperature sensor into the Gas Chamber through the temperature sensor port or through one of the built-in perfusion holes and position it in a reference well.
Features
- Chambers available for any stage brand/model including Z piezo and upright.
- Magnetic inserts for Petri Dishes, Slides and Multi Well Plates
- Magnetic locks hold Petri, Slides and Multi Well Plates in the correct position inside the chamber
- Built-in Perfusion holes
- Temperature sensor inlet port for sample feedback mode operation
- Perfusion holes available for inlet and outlet of tubes
- Sliding lid for easy access to the sample
- Optional lids are available for Koehler Illumination, laser safety and injection during imaging
- Dual gas chamber featuring two independent gas compartments for imaging under separate gas environments in the same experiment
Need a SLIM profile ?
Remove the chamber riser and use the Koehler Lid.
Perfusion ?
Each chamber features up to 12 holes for inlet and outler of perfusion tubings
Different sample holder ?
Change the chamber insert. It is a 5 seconds task with the new magnetic sample holders
Keep the sample in the correct position by using the Magnetic Locks
Chambers available for any XY stage / piezo insert
| Manufacturer | Model or stage opening | Climate Chamber | Fixing tool | ||||||||||||||||||||
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| Applied Precision | 51-603288-000 Delta vision Stage | H201-K-FRAME | DV-PA | ||||||||||||||||||||
| ASI | 5MS-2000, MS-2000 FT, PZ-2000, PZ-2000FT | H201-K-FRAME | - | ||||||||||||||||||||
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| Mad City Labs | Z100/Z500 Piezo stages | H201-MCL-Z100/500 | - | ||||||||||||||||||||
| Marzhauser | Scan IM series (110x160mm opening) | H201-K-FRAME | - | ||||||||||||||||||||
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| Thorlabs | Thorlabs MLS203-1 /MLS203-2 | H201-K-FRAME | THORPA | ||||||||||||||||||||
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| Upright Microscopes | any stage for uprightmicroscopes | H201-UP | - | ||||||||||||||||||||
CO2 - O2 CONTROLLERS
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and or O2 accuracy is ±0.1% with adjustable total output flow rate.
A vibration free humidity module is typically used with a Cage Incubation System. When Humidity control with feedback is required, the Active Humidity Controller can be connected to the Bold Line Gas Controller.
Features
- CO2 accuracy: 0.1% (see Controller page for more info)
- O2 accuracy: 0.1% (see Controller page for more info)
- Touch Screen Interface - OKO-TOUCH
- Output Flow Rate: adjustable from OKO-TOUCH
- Compatible with Active Humidity Controller
- Programmable CO2 (and /or) O2 cycles
- Compatible with SMART BOX for data logging and remote operation - Optional
- Data Logging via OKOLAB Data Log Software - Optional
- Compatible with: NIS Elements, MetaMorph, Micromanager
Preventing evporation?
Use the best Humidifier on the market: ACTIVE HUMIDITY CONTROLLER
It connects to the Gas Controller and shows up in OKO-TOUCH
Hypoxia and / or Hyperoxia ?
Oxygen control is our pride.
Check out the available solutions in the Controller List
Multiple units ? Be Smart - go central
One controller feeds up to 10 microscope incubators.
Microscopes can be in different rooms.
Available Bold Line Digital Controllers
| CO2 | O2 | Accuracy | Outlet P, barg (psi) | |
|---|---|---|---|---|
| CO2-Unit-BL | 0-18% | - | ±0.1% | 0 |
| CO2-Unit-BL-CP2 (multi-user centralized controller) | 0-10% | - | ±0.1% | 1.5 (22) |
| O2-Unit-BL [1-20] | - | 1-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10;1-18] | 0-10% | 1-18% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-20;1-95] | 0-20% | 1-95% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10; 0-1] | 0-10% | 0-1% | ±0.1% | 0 |
CO2 - O2 MANUAL GAS MIXERS
These gas mixers employ accurate floating ball flow meters to mix gas AIR and CO2 streams. The desired gas concentration is achieved by measuring the individual flow rated prior to mixing.
For Hypoxia or Hyperoxia applications, a third flow meter if added to measure N2 or O2 flow rate.
No feedback is present on gas concentration.
Features
- 2GF-MIXER mixes AIR with 100% CO2
- CO2 range: 0-15%; accuracy ±1%
- 3GF-MIXER-HYPOXIA mixes AIR, CO2 and N2
- CO2 range: 0-15%, accuracy ±1%
- O2 range: 2-20%, accuracy ±1%
- 3GF-MIXER-HYPEROXIA mixes AIR, CO2 and O2
- CO2 range: 0-15%, accuracy ±1%
- O2 range: 40-85%, accuracy ±1%
- Filtering device: PTFE membrane with 0.2 mm pores
- Compatible with OKO-AP, if compresses air is not available
- For Humidity Control, add Vibration Free Humidity Module
- Not compatible with Active Humidity Controller
Working with Premixed gas ?
All you need is a single flow meter to regulate the flux
Hypoxia and / or Hyperoxia ?
3GF-MIXER is a simple and cost effective solution also for these application.
No Compressed Air ?
Use AIR-PUMP to feed air into the Manual Gas Mixers
Available Manual Gas Mixers
| 2GF MIXER | 0-20% | - | 0 |
| 3GF MIXER-HYPOXIA | 0-15% | 2-20% | 0 |
| 3GF MIXER-HYPEROXIA | 0-15% | 40-85% | 0 |
VIBRATION FREE HUMIDITY MODULE
The Cage Incubator is equipped with a vibration-free gas humidifier which employes a semi-permeable membrane. Humidification occurs without bubbling to ensure vibration-free operation.
A water trap is located between the humidifier and the gas chamber preventing any water droplet from accidentally reaching the sample.
Features
- Gas is humidified through a semi-permeable membrane
- No bubbling, i.e. no vibrations
- Fits inside the enclosure
- Re-filler without opening the enclosure and without perturbing the sample
- If active control of humidity is needed, see HM-ACTIVE
OKO-TOUCH
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
It allows to:
- Turn all Bold Line Controllers on/off
- Define set point values
- Set flow rates of the Temperature Unit
- Set flow rates gas controller
- Define gas cycles of gas controller
- Select sample or enclosure feedback mode
- Display all set points and measured parameters
- Run self calibration procedures
SMART BOX
Smart Box connects to the Bold Line Controller and provides the following features:
Features
- Data Logging
- Remote Operation of the incubator via Internets, Tablet or SmartPhone
- Video Streaming server
- Remote assistant
